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Siberian Mouse Hd 132 20
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The present study has been undertaken to identify the source of new oocytes in the postnatal mouse ovary and to determine whether primordial follicle numbers do or do not persist over a period of time when oogenesis is active.
With increasing age, BrdU-labeled cells in the neonatal mouse ovary were found to be distributed throughout the interstitial connective tissue of the cortex, with the exception of primordial follicles, which only contained BrdU-labeled cells in the germinal epithelium of the stage 4 oocyte.
As a first step in identifying the source of new oocytes, we used a combination of BrdU incorporation, immunohistochemistry and morphometric analysis to localize and quantify cells replicating DNA in the ovary. This approach has been used successfully in the past to identify the source of new oocytes in the adult mouse (Spradling et al. 2003) and in the adult human ovary (Spradling et al. 2004).
This distribution of BrdU-labeled cells was found to persist in 5-week-old adult ovaries. However, these BrdU-labeled cells were not found within the primordial follicle niche. Hence, the source of new oocytes and primordial follicles in the adult mouse ovary is restricted to the germ cells of growing follicles in the antral and preantral follicle stages. At the same time, a subset of BrdU-labeled cells was found within the oocytes of all preantral follicles, suggesting that some primordial follicles are in a state of persistent replication.
We can only hypothesize as to the mechanisms responsible for the maintenance of a pool of cells capable of dividing. Many of the factors known to promote cell division and oogenesis are present in the normal female mouse, including the presence of gonadotropins, the positive growth factor IGF-1 and the presence of oocytes with large nuclei and increased cytoplasmic organelles that are capable of synthesizing DNA (Cohen 1979, Ossovsky & Crain 1971, Ngo & Barash 1978). In addition, the preantral follicles are continually replenished by small numbers of primordial follicles.
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